Two undergraduate students, two alums and two professors from Bellevue University’s biology department recently uncovered a new way for immune cells to control antiviral responses. The work involves a collaboration with Dr. Tom Petro at UNMC and was published in FEBS Letters.
Danielle Baldi and Sierra Athen, who are current students at Bellevue University, and Shawn Freed and Jason Snow, who are now alumni, first started the project in 2018. Associate Professor of Biology Dr. Tyler Moore and Program Director of Biology Dr. Scott Pinkerton also worked on the project.
“The same immune responses that fight viruses also cause damage to our own tissues, so it is important for immune cells to respond no more than necessary,” Moore said. “Our findings show a new way for cells to determine the magnitude of antiviral immune responses.”
The work on the project took place over several years; with classes of students focusing on it and then handing the project off to subsequent students to continue the research.
“The project required culturing live cells, stimulating them with viruses, manipulating the genes of these cells and measuring activation of gene expression,” Moore said. “Students also needed to do a lot of problem solving to optimize experimental conditions and draw conclusions from the data.”
Freed was the first to observe the interesting phenomenon of hyperactive antiviral immune responses in cells when certain pathways were inhibited, Moore said. Freed and others in the group subsequently went on to characterize the conditions when the specific phenomena occurred. Then students worked together over the next three years in different ways to test their hypotheses.
“Essentially, we found that this well-studied pathway blocks antiviral immune responses and we can restore those antiviral responses by blocking that pathway,” Moore said. “By understanding how cells determine the magnitude of responses, it might be possible to develop therapeutics to tailor the immune response to the particular virus infections.”
The paper was also recently selected as a highlight on the FEBS Letters website for being a paper “that contributes significantly to the research field.” The paper is featured on the slider of the FEBS Letters homepage.
*Story by Krystal Sidzyik
Fabiola Aviles, one of our former Biology majors, is the lead author on an article describing the discovery of a new bacterial species. The species was isolated from the San Elijo Lagoon near San Diego, where I took some samples while attending a science conference in 2019. After returning back to the lab, one of the samples gave bright yellow colonies on the plates. Fabiola extracted its DNA and sequenced the genome of this bacterium to find out its identity and characteristics.
At first glance we didn’t think we had anything unique there, because it looked like other soil bacteria, however Fabiola insisted on including it in her thesis project at BU, which made us look a little closer at the genome, and we were surprised to find it was a rather unique species.
As it turns out, the bacterium has the ability to decompose chitin (which is the hard outer shell of lobsters, shrimp and crabs, and insects), and cellulose, but can also feed on an unusual sugar called fucose. Therefore, the species was named Cellulosimicrobium fucosivorans (which literally means ‘fucose eating’ microbe). Fucose is found in insect and mammalian guts, but also in plant and algal polysaccharides. The ability to metabolize fucose gives certain bacteria in the gut flora a competitive advantage for colonization and invasion, making them pathogenic. Little is known about the ability to metabolize this sugar in nature.
It turned out to be even more surprising when we found out that the ability to feed on fucose was linked to the yellow color of the bacteria. Without the fucose, the bacteria are a pale white-pink color, but produce yellow carotenoids when fucose is present. These carotenoids are known to protect species from UV radiation damage from the sun (kind of like an internal sunscreen).
If you think about the environment this bacterium lives in, this starts making sense. There is plenty of algal and plant material, and if a bacterium can take the advantage of feeding on a component that others don’t use (for example fucose), then it has a competitive advantage. In addition, the coastal lagoons in San Diego gets lots of sun exposure and producing your own sun protection (carotenoids) seems a necessity for survival. So, this species turns out to be well adapted to living in the coastal sunny lagoon area.
The findings and a full detailed description were recently published in Archives of Microbiology and can be accessed here: https://rdcu.be/cmTYq
This is not Fabiola’s first scientific achievement. During her undergraduate studies at BU she also published a genome paper on Thiorhodococcus bacteria and recently completed a metagenomic study of the Piñones Lagoon in her home state of Puerto Rico. Fabiola plans to continue her studies in the field of marine biology, and these publications will undoubtedly help her in building out a successful scientific career.
This is project is a perfect example of how it is important to have an understanding of the different scientific disciplines, like microbiology, biochemistry, genomics, evolution, and bring them together to make unique discoveries.
You may not realize that, over 100 million years ago, Nebraska was covered by a large sea. This covered an estimated 20,000 acres, but now all that is left is salt marshes that are widespread around the state.
These salt marshes are part of a rare wetland type that occurs in the Sandhills, the North Platte Valley and the valley of the Salt Creek and Little Salt creek. These saline wetlands are in danger of disappearing and several conservation efforts are ongoing to preserve this unique ecosystem.
Restoration and conservation efforts of native ecosystems for the most part focus on the insect and plant community, however the underlying microbial community is crucial to any preservation of marsh vegetation. Little is known about the bacteria and nutrient metabolism that occurs in these inland salt marshes, that are thousands of miles away from any coast, and have not been part of a larger saline body of water for apparently millions of years.
“Microbes are responsible for cycling and balancing the nutrients in any environmental ecosystem. So if we try to study and preserve these unique wetlands, we should start at the core, and look at what microorganisms are present and how they metabolize nutrients” says Dr. John Kyndt, who led the current research effort.
The BU team set out to investigate the algal and bacterial composition (the so-called ‘microbiome’) of these Salt Marshes. Samples were taken from the Salt Creek marshes near Lincoln last fall and analyzed in the BU labs. Biology undergraduate student Sierra Athen extracted DNA and used Illumina sequencing to produce the bacterial signatures. Together with BU employee Shivanghi Dubey, they used bioinformatic analyses to identify and compare the algae and bacteria in these samples. The results were recently published in the journal Life:
“These marshes are one of the only places in the United States where the naturally occurring water is saline. Realizing that this study could help in conservation of these endangered salt marches was not only motivating but also quite intriguing”, says Shivangi Dubey.
The team found that all these bacteria are well adapted to the high saline and alkaline conditions of the marshes, but some rare bacteria are present as well. For example, Rubribacterium was previously only isolated from an Eastern Siberian soda lake, but appears to be one of the most abundant bacteria present in the Nebraska Salt Marsh samples.
The microbial ecosystem appears to be well-balanced as far as sulfur and other nutrients, nevertheless, urban development, agricultural runoff, and the growth of invasive plant species are some of the current threats to maintaining these vital ecosystems. This current study establishes a good baseline for further studies on microbiological diversity, nutrient cycling, and ecological impacts in this locally important watershed.
“These land-locked saltmarshes are a relic of ancient oceans and, in some way, studying these salty microbes that live there, takes you back to the last ages of dinosaurs, which can be quite fascinating”, says Dr. Kyndt.
It is not every day that an entire bacterial genus is discovered and reclassified. We are lucky when occasionally we find a new species, but that alone certainly does not redefine an entire genus. It took the sequencing and comparative analysis of eight new genomes, in addition to the three known genomes of heliobacteria, to come to this discovery.
The family of heliobacteria are a small group of phototropic bacteria that were serendipitously found while Dr. Howard Gest was teaching a lab class in microbiology some 38 years ago. It is therefore suiting that an undergraduate student, Dayana Montano Salama, was a key player in this new research that led to new genus of Heliomicrobium.
“A combination of genome sequencing, whole genome comparison and detailed genetic analysis of the 11 species was performed to reclassify them into the Heliobacterium and Heliomicrobium genera.” says Dr. John Kyndt, who was the lead on this research project. “In the end, the new genus Heliomicrobium includes four unique species, although more will undoubtedly be discovered in the future with growing metagenomic efforts.”
The new genus name comes from the Greek words helios sun; micros small; bios life, so Heliomicrobium literally means ‘sun microbe’. Heliobacteria have been recognized as the ancestors of photosynthesis in the evolution of photosynthetic bacteria. All heliobacteria are strictly anaerobic, endospore-forming bacteria, and the ability to form endospores is unlike all other phototrophic bacteria, but similar to species of Clostridium and Bacillus. An improved understanding of the taxonomic classification of these bacteria will ultimately lead to a better understanding of the evolution of photosynthesis in bacteria in general.
The research was an international collaboration with Dr. Johannes Imhoff from the GEOMAR institute in Germany, and was recently published in the International Journal of Systematic and Evolutionary Microbiology, which is the flagship scientific journal for bacterial taxonomy.
A link to the publication can be found here: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004729
What do you get when you put a project manager with an electrical & electronics engineering degree, and a biochemist together in a lab? It sounds like the beginning of a bad joke, but nothing is further from the truth. Sometimes unusual interdisciplinary collaborations can turn out to be very productive.
This is exactly what happened when Shivangi Dubey, a BU employee in the IT Department, reached out to Dr. John Kyndt at the Science Department, and expressed that she had an interest in working on some real-life science projects. Together they started working on some of the ongoing project of bacterial genome sequencing at the BU Science Labs.
The collaboration turned out to be fruitful very fast. “Shivangi showed an honest interest and curiosity in the project and was very driven to accomplish results” says Dr. Kyndt. “From the start she described herself as a science enthusiast and a constant leaner. That made it real easy to teach her basic lab and bioinformatic skills”.
In less than two months after the start of the project, the team was able to publish a new article on the genome analysis of an unusual bacterial species. The publication was just released online in Microbiology Resource Announcements this week: Genome Sequence of the Unusual Purple Photosynthetic Bacterium Phaeovibrio sulfidiphilus, Only Distantly Related to Rhodospirillaceae, Reveals Unique Genes for Respiratory Nitrate Reduction and Glycerol Metabolism
Not only did they complete the genome of this species, the genomic analysis and comparisons also revealed the genetic reason behind the strict anaerobic nature of this bacteria and revealed unique metabolic pathways that will be the basis for further physiological studies.
“It all started when a research article on “Photoactive Proteins” by Dr. Kyndt caught my attention and out of curiosity I reached out to Dr. Kyndt to learn more about his research. He not only addressed my curiosity but also provided me an opportunity to learn from him and partner with him in his next research project. Dr. Kyndt has been very supportive and provided guidance throughout this research. I look forward to many more such opportunities to learn from him” Shivangi.
In an effort of continuous education, both Shivangi and Dr. Kyndt are currently taken an online course in Bacterial Bioinformatics, and are hoping to complete their Certificate in Bioinformatics from the University of Virginia in a couple of weeks.
The giant phototrophic bacterium Thiospirillum jenense was first discovered in 1838. Its large size (up to 100μm), spiral shape, orange-brown color, and formation of sulfur globules visible under the light microscope, made it an interesting study object for several renowned microbiologists throughout the 20th century.
Although a very intriguing organism, it was proven to be extremely difficult to cultivate in the lab and even to date, no pure culture has been obtained. For a long time, the only source of this bacterium was a pond in former East Germany where it grew April to July, and bacterial cultures had to be smuggled across the then closed borders to West Germany. For more than five decades, the problems in cultivation of Tsp. jenense and later also the missing availability of cultures, have disabled further detailed studies.
Even though the growth and phototactic behavior was studied, nothing was known about the molecular genetics of this intriguing species, not even 16S rRNA sequences, so no complete taxonomic classification could be performed. Up until now.
Illumina-based sequencing was performed at Bellevue University on an enriched sample of Thiospirillum jenense, obtained from Dr. Johannes Imhoff from the GEOMAR Ocean Research Center in Kiel (Germany). Genome sequencing and metagenomic binning analysis now provided the full genome of Thiospirillum jenense which was published just this week in Archives of Microbiology. It showed the unique placement of this species amongst the purple sulfur bacteria and, in addition, potentially resolved some of the genetic reasons behind the challenges of cultivating Tsp. jenense that have been limiting further experiments in the past.
One key component appears to be lack of a high-affinity oxidase (FixNOP), which presumably renders the cells highly sensitive to oxygen damage. In addition, the two sequenced contaminant species, Rhodopseudomonas palustris and a new species Rhodoferax jenense, might help with removal of oxygen in the cultures. These results likely explain the difficulties with obtaining pure cultures of Tsp. jenense as described in the paper, and opens up the doors for new cultivation methods.
Having genomic and genetic data available for Tsp. jenense has widened our understanding of the microbial diversity and will undoubtedly help to further identify similarly unique species in environmental samples, where they play an important role in the sulfur cycle and other nutrient recycling in the environment.